Proteinase K is a proteolytic enzyme. The enzyme uses Ca2+ ions for structural stability. Due to the high total activity of the enzyme, lysis can be carried out in the presence of EDTA. Proteinase K is therefore excellently useful to digest contaminating proteins and improve the extraction of nucleic acids from various biological samples.
Recommended Use / Scope
Minerva Biolabs Venor®GeM Sample Preparation Kit is designed for highly efficient extraction of DNA from standard samples, like cell culture supernatants containing up to 106 cells, with high efficiency. However, other samples matrices like cell pellets, serum, or fermentation products may contain proteins at a concentration higher than 10 mg/ml. These samples may require an additional treatment with Proteinase K to increase the overall DNA yield and reach highest sensitivity.
In combination with Minerva Biolabs´ Venor®GeM Sample Preparation Kit, 10 µl of the Proteinase K solution are used for each sample after it has been mixed with Conditioner (Venor®GeM Sample Preparation Kit) or Buffer A (Microsart AMP Extraction).
Cat. No. 56-0002
1 vial freeze-dried, for 50 extractions, to be dissolved in 550 µl of the included rehydration buffer.
Shelf Life and Storage
The freeze-dried product shall be stored at +2 to +8 °C. The rehydrated stock solution must be stored at < -18 °C. Avoid repeated freezing and thawing. Preparation of aliquots might be required if more than 5 freezing/thawing cycles are required.