Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination by PCR.
Type of PCR
For conventional, endpoint PCR
Recommended Use / Scope
Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics.
Primer sets, nucleotides and polymerase at optimized concentrations lyophilized in one tube
10x reaction buffer
Lyophilized positive control DNA
Lyophilized internal amplification control
Primer sets, nucleotides and polymerase are prepared in aliquots of 25 tests.
- Cat. No. 11-1025G 25 Tests
Cat. No. 11-1050G 50 Tests
Cat. No. 11-1100G 100 Tests
Cat. No. 11-1250G 250 Tests
Gel electrophoresis at endpoint of PCR
PCR reaction tubes
Optional for process validation and EP 2.6.7 compliant testing: Internal Control DNA extra (4 vials for 300 µl each of internal amplification control; Cat. No. 11-1905) 10CFU™ Sensitivity Standards available for all EP listed mycoplasma species
Required lab devices
Regular PCR cycler
Agarose gel electrophoresis and DNA staining system
Shelf Life and Storage
Components are maintainable at +2 to +8 °C for at least 6 months. After rehydratisation the reagents must be stored at -18 °C.
EP 2.6.7 compliance
Use of Venor®GeM Classic for QA testing of biologicals like master and working cell banks, autologous cells, culture media, bulk harvest and final product testing according to EP 2.6.7 is applicable after appropriate sample preparation and process validation.
Fig. Amplified PCR products are visualized by standard gel electrophoresis.