PCR amplification has become an indispensable method in every molecular biology lab.
PCR amplification has become an indispensable method in every molecular biology lab. Usually, the common reaction components (e.g. buffer, dNTPs, MgCl2, and DNA polymerase) are mixed together by lab operators to create a master mix, which is then aliquoted to individual reaction tubes. Reaction-specific primers and template DNA are added prior to PCR, adding further pipetting steps to the procedure, and thus increasing the margin of error, the risk of cross-contaminations and work time. The application of ConviFlex™ DNAmp Mix requires minimal hands-on time and reduces the risk of cross-contaminations due to the minimal number of pipetting steps required to set up the PCR reaction.